Wireless optoelectronic photometers for monitoring neuronal dynamics in the deep brain

Luyao Lu, Philipp Gutruf, Li Xia, Dionnet L. Bhatti, Xinying Wang, Abraham Vazquez-Guardado, Xin Ning, Xinru Shen, Tian Sang, Rongxue Ma, Grace Pakeltis, Gabriel Sobczak, Hao Zhang, Dong-oh Seo, Mantian Xue, Lan Yin, Debashis Chanda, Xing Sheng, Michael R. Bruchas and John A. Rogers. Proceedings of the National Academy of Sciences of the United States of America, Volume 115, Issue 7, 13 February 2018, Pages E1374-E1383 Read More

Abstract

Capabilities for recording neural activity in behaving mammals have greatly expanded our understanding of brain function. Some of the most sophisticated approaches use light delivered by an implanted fiber-optic cable to optically excite genetically encoded calcium indicators and to record the resulting changes in fluorescence. Physical constraints induced by the cables and the bulk, size, and weight of the associated fixtures complicate studies on natural behaviors, including social interactions and movements in environments that include obstacles, housings, and other complex features. Here, we introduce a wireless, injectable fluorescence photometer that integrates a miniaturized light source and a photodetector on a flexible, needle-shaped polymer support, suitable for injection into the deep brain at sites of interest. The ultrathin geometry and compliant mechanics of these probes allow minimally invasive implantation and stable chronic operation. In vivo studies in freely moving animals demonstrate that this technology allows high-fidelity recording of calcium fluorescence in the deep brain, with measurement characteristics that match or exceed those associated with fiber photometry systems. The resulting capabilities in optical recordings of neuronal dynamics in untethered, freely moving animals have potential for widespread applications in neuroscience research. © 2018 National Academy of Sciences.All Rights Reserved.

Full Text

.

Posted on February 22, 2018
Posted in: Axon Injury & Repair, Clocks & Sleep, Neurogenetics & Transcriptomics Authors: