Start-up and Shutdown Procedures

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Start-Up and Shutdown Procedures

Been away from the scope too long? Stubborn independent streak? Need to take images, and need them now? Look no further. Presented here in easy to read form are some quick to follow steps to get you up and on your way to vibrant, beautiful images in no time.

  1. log on to the computer using your network account and password
  2. power on microscope & components
  3. turn Chameleon key from Standby to On (if using Chameleon)
  4. start “LSM 510” software
  5. make sure “scan new images” is selected and click “start expert mode”
  6. turn on appropriate laser lines in the “laser” panel
  7. setup configuration by either:
    1. opening an old image and selecting “reuse”
    2. click “store/apply” configuration
  8. select “Fast XY” and configure mode settings
  9. select “Single”

Complementary to the Quickstart guide is this Quickfinish. Here are the few short steps to make sure nothing is damaged or lost in the shutdown process.

  1. save your data
  2. make sure your data is saved
  3. exit the Zeiss software
  4. if you used the Argon 488-458-514 laser, wait until it completes the cooldown process (you can tell by placing your hand above the fan, if there is a strong breeze, wait longer)
  5. if you used the Chameleon laser, switch key from “On” to “Standby”
  6. power off the microscope & components
  7. logout