Lentiviral Constructs

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Available Vectors

Vector Promoter Transgene Expression – Cell Culture Expression – in vivo
FCIV Ubiquitin Venus YFP (IRES) Murine neocortical neurons and astrocytes Expressed in neurons and other cell types; maximal expression may take >7 days
CMV-GFP CMV GFP SH-SY5Y, murine neocortical astrocytes>>neurons; expression in neurons takes ~7 days Expression in astrocytes and oligodendrocytes after injection into corpus callosum (McIver, 2005)
CMV-lckGFP CMV Membrane-targeted GFP Same as CMV-GFP
PGK-GFP PGK (phosphoglycerate kinase) GFP Murine neocortical neurons > astrocytes Expressed in neurons and other cell types; maximal expression may take 5-7 days
PGK-lckGFP PGK Membrane-targeted GFP Murine neocortical neurons> astrocytes
MBP-GFP MBP (myelin basic protein) GFP Murine oligodendrocytes, possibly other astrocytes Oligodendrocyte expression within 3 days (McIver 2005)
MBP-lckGFP MBP Membrane-targeted GFP Murine oligodendrocytes, possibly other astrocytes
SYN-GFP Synapsin GFP Murine neocortical neurons
GFAP-GFP glial fibrillary acidic protein GFP Murine cortical astrocytes
MND-GFP myeloproliferative sarcoma virus enhancer (Halene et al., 1999) GFP Murine neocortical astrocytes>>neurons; neuronal expression takes ~7 days
shRNA vectors
pLKO.1 U6 

CMV

shRNA 

puromycin

U6-PGK-GFP U6 

PGK

shRNA 

GFP

U6 promoter expresses in all cell types; reporter gene (GFP) is expressed differentially – see notes above

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