Lentiviral Constructs

Available Vectors

VectorPromoterTransgeneExpression – Cell CultureExpression – in vivo
FCIVUbiquitinVenus YFP (IRES)Murine neocortical neurons and astrocytesExpressed in neurons and other cell types; maximal expression may take >7 days
CMV-GFPCMVGFPSH-SY5Y, murine neocortical astrocytes>>neurons; expression in neurons takes ~7 daysExpression in astrocytes and oligodendrocytes after injection into corpus callosum (McIver, 2005)
CMV-lckGFPCMVMembrane-targeted GFPSame as CMV-GFP
PGK-GFPPGK (phosphoglycerate kinase)GFPMurine neocortical neurons > astrocytesExpressed in neurons and other cell types; maximal expression may take 5-7 days
PGK-lckGFPPGKMembrane-targeted GFPMurine neocortical neurons > astrocytes
MBP-GFPMBP (myelin basic protein)GFPMurine oligodendrocytes, possibly other astrocytesOligodendrocyte expression within 3 days (McIver 2005)
MBP-lckGFPMBPMembrane-targeted GFPMurine oligodendrocytes, possibly other astrocytes
SYN-GFPSynapsinGFPMurine neocortical neurons
GFAP-GFPglial fibrillary acidic proteinGFPMurine cortical astrocytes
MND-GFPmyeloproliferative sarcoma virus enhancer (Halene et al., 1999)GFPMurine neocortical astrocytes>>neurons; neuronal expression takes ~7 days
shRNA vectors
pLKO.1U6 
CMV
shRNA
puromycin 
U6-PGK-GFPU6 
PGK
shRNA 
GFP
U6 promoter expresses in all cell types; reporter gene (GFP) is expressed differentially – see notes above

Back to the Hope Center Viral Vectors Core webpage.