In Vitro Physiology

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Overview

The In vitro Physiology core provides assessment of synaptic function in rodent models using physiological approaches such as in vitro brain slice preparations and single-synapse studies in dissociated cell culture.

Location

Biotech Building, Room 316

Services

  1. Primary Cultures, Transfected Cells
    • Patch-clamp amplifiers mounted to inverted phase-contrast microscope. Suitable for whole-cell recording from cultured neurons or transfected cells in 35 mm culture dish
    • Computer controlled local drug perfusion
    • Data analysis software
    • Studies available:
      • Glutamate (excitatory) and GABA (inhibitory)-mediated synaptic transmission
      • Responses to exogenous glutamate and GABA
      • Action potential generation
  2. Brain slices
    • Extracellular amplifiers mounted on stereomicroscope. Suitable for brain slice recording of field EPSPs, synaptic plasticity studies in hippocampus.
    • Data analysis software
    • Studies available (hippocampal slices)
      • Studies of glutamate-mediated synaptic transmission
      • Baseline synaptic responses
      • Input-output curves
      • NMDA receptor and AMPA receptor-mediated transmission
      • Synaptic plasticity:
      • Paired pulse plasticity
      • Long-term plasticity (LTP, LTD)

Usage

The in vitro physiology core is open to all Washington University Neuroscience investigators through the P30 Neuroscience Blueprint Core Grant. Neuroscience investigators from nonprofit/academic institutions outside Washington University may be able to use Core Services as well.

How to Obtain Core Services

Contact Dr. Steven Mennerick, Core Coordinator, to discuss your needs.

Staff

  • Charles Zorumski, MD – Faculty Supervisor
  • Steve Mennerick, PhD – Faculty Co-Director
  • Jenny Shu – Core Staff (cell culture and transfected cell recordings)
  • Yuki Izumi – Core Staff (hippocampal slice studies)

Support/Acknowledgements

This Core is supported by a Neuroscience Blueprint Core grant (P30 NS057105) from NIH to Washington University.

Biotech Building, Room 316

Services

  1. Primary Cultures, Transfected Cells
    • Patch-clamp amplifiers mounted to inverted phase-contrast microscope. Suitable for whole-cell recording from cultured neurons or transfected cells in 35 mm culture dish
    • Computer controlled local drug perfusion
    • Data analysis software
    • Studies available:
      • Glutamate (excitatory) and GABA (inhibitory)-mediated synaptic transmission
      • Responses to exogenous glutamate and GABA
      • Action potential generation
  2. Brain slices
    • Extracellular amplifiers mounted on stereomicroscope. Suitable for brain slice recording of field EPSPs, synaptic plasticity studies in hippocampus.
    • Data analysis software
    • Studies available (hippocampal slices)
      • Studies of glutamate-mediated synaptic transmission
      • Baseline synaptic responses
      • Input-output curves
      • NMDA receptor and AMPA receptor-mediated transmission
      • Synaptic plasticity:
      • Paired pulse plasticity
      • Long-term plasticity (LTP, LTD)/

Usage

The in vitro physiology core is open to all Washington University Neuroscience investigators through the P30 Neuroscience Blueprint Core Grant. Neuroscience investigators from nonprofit/academic institutions outside Washington University may be able to use Core Services as well.

How to Obtain Core Services

Contact Dr. Steven Mennerick, Core Coordinator, to discuss your needs.

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