In Vivo Physiology

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Location

202 Biotechnology Building

Services

  • Surgical Suite

    Sterile instruments, isoflurane anesthesia, stereotaxic frame with microdrill and micromanipulators for stereotactically placing electrodes.

  • Recording Equipment

    Hard wired system for recording electroencephalogram (EEG), electromyogram (EMG) or evoked potential (EP) signals from rodents. Surgical implantation of recording electrodes is required. Amplified signals are recorded to PC. Long-term recording for hours to days is feasible. Video recording combined with EEG recording can be used to correlate behavior with electrophysiological data (e.g., are behaviors associated with electrographic seizures? Is background EEG activity and sleep architecture normal?).

  • Data Analysis Software

    Axon Instruments PClamp software is used for acquisition and analysis of EEG, EMG and evoked potential data.

Types of studies available:

  • Mice

    Video-EEG recording

    2-channel EEG or 1 channel EEG, 1 channel EMG (e.g., for sleep assessment). Determine whether behaviors are consistently correlated with electrophysiologically confirmed seizure activity on EEG (events/behaviors must be frequent enough and stereotyped enough to be able to be captured during recordings). Examine EEG background activity, sleep architecture as an assessment of brain function.

    - In anesthetized mice: evoked synaptic responses from dentate granule cell layer using bipolar electrical stimulation of the perforant pathway.

  • Rats

    - Long-term video-EEG recording

    2-channel EEG or 1 channel EEG, 1 channel EMG (e.g., for sleep assessment). Determine whether behaviors are consistently correlated with electrophysiologically confirmed seizure activity on EEG (events/behaviors must be frequent enough and stereotyped enough to be able to be captured during recordings). Examine EEG background activity, sleep architecture as an assessment of brain function.

    - In anesthetized rats: evoked synaptic responses from dentate granule cell layer using bipolar electrical stimulation of the perforant pathway. Tetanus-induced long-term synaptic potentiation at perforant pathway synapses.

    - In awake freely moving rats: evoked synaptic responses from dentate granule cell layer using bipolar electrical stimulation of the perforant pathway. Assessment of tetanus-induced long-term synaptic potentiation in awake rats.

Usage

The in vivo physiology core is open to all Washington University Neuroscience investigators through the P30 Blueprint Grant; Hope Center investigators may be eligible for additional support for Core services through the Hope Center. Neuroscience investigators from nonprofit/academic institutions outside Washington University may be able to use Core Services as well.

How to Obtain Core Services:

Contact Kelvin Yamada, Core Coordinator, to discuss your needs.

Staff

Support/Acknowledgements

This Core is supported by a Neuroscience Blueprint Core grant (P30 NS057105) from NIH to Washington University.

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