Vector | Promoter | Transgene | Expression – Cell Culture | Expression – in vivo |
FCIV | Ubiquitin | Venus YFP (IRES) | Murine neocortical neurons and astrocytes | Expressed in neurons and other cell types; maximal expression may take >7 days |
CMV-GFP | CMV | GFP | SH-SY5Y, murine neocortical astrocytes>>neurons; expression in neurons takes ~7 days | Expression in astrocytes and oligodendrocytes after injection into corpus callosum (McIver, 2005) |
CMV-lckGFP | CMV | Membrane-targeted GFP | Same as CMV-GFP | |
PGK-GFP | PGK (phosphoglycerate kinase) | GFP | Murine neocortical neurons > astrocytes | Expressed in neurons and other cell types; maximal expression may take 5-7 days |
PGK-lckGFP | PGK | Membrane-targeted GFP | Murine neocortical neurons> astrocytes | |
MBP-GFP | MBP (myelin basic protein) | GFP | Murine oligodendrocytes, possibly other astrocytes | Oligodendrocyte expression within 3 days (McIver 2005) |
MBP-lckGFP | MBP | Membrane-targeted GFP | Murine oligodendrocytes, possibly other astrocytes | |
SYN-GFP | Synapsin | GFP | Murine neocortical neurons | |
GFAP-GFP | glial fibrillary acidic protein | GFP | Murine cortical astrocytes | |
MND-GFP | myeloproliferative sarcoma virus enhancer (Halene et al., 1999) | GFP | Murine neocortical astrocytes>>neurons; neuronal expression takes ~7 days | |