Viral Vectors for Optogenetics

Suitable for in vitro applications

Vectors available for purchase

The Hope Center Viral Vectors Core is pleased to announce availability of AAV vectors for Optogenetics. These vectors were chosen for production by the Viral Vectors Core based on responses to a survey last summer, and now are available for purchase for $200/aliquot (or $128/aliquot for Hope Center faculty members). The titer for each is at least 1 x 10^13 vg/ml.

  • AAV5-EF1a-DIO-hChR2(H134R)-EYFP**
  • AAV5-EF1a-DIO-hChR2(H134R)-mCherry**
  • AAV5-EF1a-DIO-eArch3.0-EYFP**
  • AAV5-FLEX-ArchT-tdTomato

Viruses can be used for in vitro Optogenetics (or in vivo applications in your lab). The Hope Center is developing in vivo Optogenetics as a core service – stay tuned!

MTAs and Acknowledgements

Deisseroth vectors

Vectors marked ** were created by Karl Deisseroth and we are pleased to stock these constructs. First-time users must obtain an MTA directly from Stanford. Click here for instructions on how to obtain an MTA.

tdTomato vector

The tdTomato vector was created by Edward Boyden (MIT), and generated by the Hope Center Viral Vectors Core using a plasmid from Addgene. An MTA is not required for the tdTomato vector. When publishing results based on use of this vector, please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

Suggested language for Materials & Methods:

pAAV-FLEX-ArchT-tdTomato was a gift from Edward Boyden (Addgene plasmid # 28305)

Suggested language for References:

A high-light sensitivity optical neural silencer: development and application to optogenetic control of non-human primate cortex. Han X, Chow BY, Zhou H, Klapoetke NC, Chuong A, Rajimehr R, Yang A, Baratta MV, Winkle J, Desimone R, Boyden ES. Front Syst Neurosci. 2011;5:18. Epub 2011 Apr 13. 10.3389/fnsys.2011.00018 PubMed 21811444

Ideas for additional vectors?

If you’d like to suggest additional vectors for bulk production, please contact Mingjie Li or Joy Snider.