Flow Cytometry equipment

Beckman Coulter CytoFLEX S Flow Analyzer
Beckman Coulter CytoFLEX SRT Cell Sorter

Location

Fort Neuroscience Research Building, Room 3198

Overview

• Beckman Coulter CytoFLEX S Flow Analyzer
  • Four active lasers (405 nm, 488 nm, 561 nm, 638 nm)
  • 13 channels for fluorescence detection
  • CytoExpert Acquisition software for data acquisition and analysis

• Beckman Coulter CytoFLEX SRT Cell Sorter
  • Four active lasers (405 nm, 488 nm, 561 nm, 638 nm)
  • 15 channels for fluorescence detection
  • Features multiple configurations that can be easily set up and changed automatically

Usage

Training sessions are mandatory and can be scheduled by contacting Prabal Sharma (prabal@wustl.edu). Once training is complete, users will be able to reserve time on the online calendar, and will receive a personal account to access the equipment. PLEASE NOTE, a completed Sample Request Form must be submitted at least two weeks in advance of equipment use, via email to Prabal Sharma, prabal@wustl.edu.

• Flow Analyzer: The training session may be attended by 1-3 people, taking about 4 hours. The mandatory session covers instrument operation and includes creation of a full panel experiment.
• Cell Sorter: The training session may be attended by 1-2 people and takes 1-2 days. The mandatory session covers instrument operation, cell sorting procedure and a supervised practice experiment.