DNA/RNA Purification Core

Isolation of nucleic acids from diverse biospecimen samples Read More

Notice re Hope Center Subsidy

Beginning with the August 2013 billing cycle, the Hope Center subsidy will apply only if the billing PI is a Hope Center faculty member.

About

The DNA/RNA purification core offers nucleic acid extraction from a variety of sources including fresh or frozen whole blood, buffy coat, saliva, and frozen tissue and cell pellets.

  • Large volume DNA samples (5 – 10 ml) are extracted on the Qiagen AutoPureLS using proven Puregene chemistry, a salt precipitation method that removes protein contaminants and enzyme inhibitors.  The AutoPure LS platform is capable of high-throughput purification from large volume samples (5 – 10 ml) in ~ 80 minutes (16 samples).  Purified DNA is suitable for downstream applications requiring high quality, high molecular weight DNA such as pcr, southern blot, and library construction.
  • Small volume DNA extraction (<1 ml) and RNA extraction use column based purification on a Qiacube automated workstation.
  • The Qiagility liquid handling workstation enables high-precision pipetting, eliminating manual pipetting errors and ensuring consistent, reproducible results.

Resources and Guidelines for Submitting Samples

AutopureLS – Large Volume samples

The AutoPureLS uses proven Puregene chemistry and features sample tracking, precision reagent dispensing, and controlled mixing.  Batches of 8 or 16 samples can be processed.  DNA is quantitated by spectrophotometric measurement of optical density at 260nm and 280nm wavelengths.  Quantitation by AccuBlue is also available.

Sample Guidelines for the AutopureLS:

The AutopureLS can process sample volumes of 1 – 5ml or 5 – 10ml.  Frozen tissue (50 – 100mg) or cell pellets (up to 150 million cells) can also be processed.

Blood samples should preferably be collected in EDTA (purple cap tubes).  Collection in ACD (citrate, yellow cap tubes) is also acceptable.  DO NOT USE GREEN CAP TUBES.

For best results, freshly drawn blood should be stored at 4C for less than 4 days or should be frozen at -80C on the day of collection.  Samples should never be stored at -20C as they quickly degrade.  Samples should be submitted to the core within 1 – 4 days after collection.

Tissue samples and cell pellets should be snap frozen on dry ice and stored at -80C.

Samples stored for more than 1 year or at less than -80C may result in lower yield and quality of DNA.  Multiple freeze/thaws will also affect DNA yield and quality.

All sample tubes should be labeled with the PI name and date collected.

Qiacube – Small volume samples

The Qiacube is available for DNA and RNA purification from up to 20mg frozen tissue or from frozen cell pellets (up to 5 million cells).  The Qiacube uses silica membrane spin columns for automated purification of up to 12 samples per run.  Nucleic acid quantitation by spectrophotometric analysis or by AccuBlue is available.

Sample Guidelines for the Qiacube:

Tissue samples and cell pellets should be snap frozen on dry ice and stored at -80C.  Samples should never be stored at -20C as they quickly degrade.

Samples stored for more than 1 year or at less than -80C may result in lower yield and quality of DNA.  Multiple freeze/thaws will also affect DNA yield and quality.

All sample tubes should be labeled with the PI name and date collected.

Qiagility – Liquid handling

The Qiagility is a liquid handling workstation capable of high-throughput reagent dispensing for automated DNA quantitation, serial dilutions, pcr reaction set-up (up to 96 samples in 30 minutes) and sample pooling.  High-precision pipetting eliminates manual pipetting errors and ensures consistent, reproducible results.

Acknowledging the Core

Please acknowledge the Core in manuscripts as well as posters and talks.  Suggested language: “This work was supported by the Hope Center DNA/RNA Purification Core at Washington University School of Medicine.”

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Contact Information

Inquiries and sample submission

For inquiries about a new project, contact Core Director Renate Lewis.

Inquiries

To submit samples, please complete a sample submission form and forward to Dave Carrell.

Sample submission form

Email address to submit form

Pricing

Pricing