Anil Cashikar, PhD
Research Instructor in Cell Biology & Physiology
Multivesicular body and ESCRT functions in proteostasis Read More
|Lab Phone:||(314) 747-4233|
|Lab Location:||Cancer Research Building, Room# 4619|
|Keywords:||protein aggregation, cell-to-cell transmission, exosomes, multivesicular bodies, endosomal sorting complexes required for transport (ESCRT), molecular chaperones, live-cell assays, electron microscopy|
Multivesicular body and ESCRT functions in proteostasis
The goal of my current research is to understand the importance of multivesicular bodies (MVBs; also known as late endosomes) and endosomal sorting complex required for transport (ESCRT) proteins in neurodegenerative diseases. MVBs are central to degradation of cell surface receptors, play an important role in the regulation of cell signaling and serve as progenitors for exosomes. ESCRT proteins are essential for formation of the intralumenal vesicles characteristic of MVBs. The relatively low pH, large membrane surface area and unique lipid composition inside MVBs may influence protein aggregation. MVBs may also be important in prion-like transmission of protein aggregates, a critical step in the spread of pathology in the central nervous system. Mutations in one ESCRT protein (CHMP2B) cause frontotemporal lobar dementia (FTLD) and amyotrophic lateral sclerosis (ALS). These facts underscore the need to illuminate the importance of MVBs and ESCRTs in neurodegenerative diseases. Toward this end, we are currently examining the molecular mechanism of ESCRT function using novel live cell assays, electron microscopy, and gene expression-based methods.
Last updated January 2014.